Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal
2013 , Vol 17 , Issue 2
Main spectrophotometric methods for the determination of total phenolic content and antioxidant capacity
İnönü Üniversitesi, Eczacılık Fakültesi Analitik Kimya Anabilim Dalı, Malatya, Türkiye
DOI :
10.12991/201317377
This article gives the information regarding mainly spectrophotometric methods that used to measure
antioxidant activity. Ideally, the antioxidant activity should be tested using both in vitro and in vivo techniques but due
to the high cost of in vivo testing, many products are evaluated by in vitro testing. On the basis of chemical reactions
mechanism, major antioxidant capacity assays can be divided into two categories: hydrogen atom transfer (HAT)
reaction based assay and single electron transfer (SET) reaction based assay. HAT-based assays include Oxygen
Radical Absorbance Capacity (ORAC), Total Radical Trapping Antioxidant Paramater (TRAP) and Crocin Bleaching
Assays. SET-based assays include the total phenols assay by Folin-Ciocalteu reagent (FCR), Trolox (6-hydroxy-
2,5,7,8-tetramethylchroman-2-carboxylic acid) equivalence antioxidant capacity (TEAC), ferric ion reducing antioxidant
power (FRAP), 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity (DPPH) assay and total antioxidant
potential assay using a Cu(II) complex as an oxidant (CUPRAC).
Keywords :
Antioxidant capacity, total phenolic content, hydrogen atom transfer reaction, electron transfer reaction