Ege Üniversitesi, Eczacılık Fakültesi/Farmakognozi AD, İzmir, Türkiye DOI : 10.12991/201115431 Aerial and underground parts of Galanthus woronowii Losinsk., a wild growing species in north-eastern Anatolia, were collected during flowering period. Quality control and acetylcholinesterase inhibitory activity determinations were carried out on Bulbus and Herba Galanthi prepared from plants collected from two different localities. In the context of quality control studies, contents of humidity, total ash, sulphated ash, acid-insoluble ash and total alkaloids of the drug specimens were determined and found to range between 8.463-9.343 %, 6.950-14.947 %, 9.743-17.930 %, 1.102-3.565 % and 0.247-0.499 %, respectively. Additionally, acetylcholinesterase inhibitory activity of the alkaloidal extracts prepared from the drug specimens were determined by using Thin Layer Chromatography (TLC) combined with a bioautographic assay based on in vitro Ellman method. All of the alkaloidal extracts displayed acetylcholinesterase inhibitory activity. Keywords : Galanthus woronowii, Amaryllidaceae, Quality control determination, Acetylcholinesterase inhibitory activity