Department of Pharmacognosy, Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey DOI : 10.12991/201115438 A quantitative determination of α-tocopherol in Sarcopoterium spinosum L. extracts was carried out by TLC-densitometry and HPLC-UV method. The α-tocopherol content in S. spinosum was established between 0.017210-0.023744 % (TLC-densitometry) and 0.025966-0.037212 % (HPLC-UV). The highest amount of α-tocopherol was obtained from aerial parts in fruiting period in both methods. In addition, contents of humidity, total ash and sulphated ash of plant samples were determined according to DAB 10. Keywords : Sarcopoterium spinosum, α-Tocopherol, TLC-densitometry, HPLC-UV