Glutathione is enzymatically synthesized in the lens and generally thought to play a role as a scavenger for radicals produce by oxidative stress via an oxidation-reduction system. The role of sulphur-containing compounds is thought to be associated with maintenance of the transparency of the lens Glutathione and its related enzymes were measured for cataractous human lens nucleus. Glutathione decreased progressively with development of cataract. The level of glutathione in the nucleus was analyzed in terms of the patient age groups and in terms of lens pigmentation. Results are expressed as mean ± standart deviation. We have found that glutathione and its related enzymes in the nuclei of senil cataractous patients seemed to have no correlation with age groups. The glutathione of brown-colored lenses was 0.39± 0.28 μmol/g lens, The glutathione S-transferase was 81.75± 66.07 nmol/g lens, glutathione reductase was 97.25 ± 36.0 nmol/g lens wet weight, the glutathione of yellow-colored lenses was 0.71 ± 0.66 the glutathione S-transferase was 96.93 ± 38.30 bmol/g lens, glutathione reductase was nmol/ 126.7 ± 80.0 g lens wet weight. The difference between two group means aren’t statistically significant (p>0.05). Keywords : cataract, glutathione, sulphur- containing compounds in lens