Certain pharmaceutical preparations are being manufactured based upon ruscogenin which have wide applications in phlebologic symptoms. An important source of obtaining ruscogenin is from the sub-terranean parts of Ruscus aculeatus L. The object of the presented investigations is to follow the phenophasic dynamics of the ruscogenin content by densitometric method. It has been found that the content of ruscogenin in the roots varies between 0.0026 and 0.060 %. The maximum quantity is obtained during the period of full bloom, whereas the minimum is observed during the period of the development of propagatory buds. The sapogenin content varies from 0,05 to 0,07 % in the rhizomes. The maximum quantity is generated at the time of full bloom whereas the minimum is observed at the time of the intensive growth of the new stems. These results exhibit that the most opportune period for the collection of the raw material is during the months May and December for the roots and during August and December for the rhizomes when the content of ruscogenin is the highest. Keywords : Ruscus aculeatus, steroidal sapogenins, ruscogenin, phenological phases