Hypercinis as antidepressant antiviral and photodynamically active compounds, also having chemotaxonomic importance are well known. Their occurrence in over 20 Hypericum species was investigated by TLC method. Quantitative determination of their contents was performed spectrophotometrically. It has been found that species from the primitive sections. Ascyreia, Androsaemum, Roscyna and Spachium do not contain hypericins, whereas they occur in fairly - large amounts in advanced sections Drosocarpium and Hypericum. In most species the content of pseudohypericin exceeds that hypericin. The total quantity of hypericins in the studied species varies widely: from 0.012 % (H.polyphyllum) to 0.30 & (H.barbatum). Growth dynamic studies on the medicinally importance species H. perforatum and H. maculatum establish their maximum content during flowering period. Hypericins content in different regional samples vary from 0.07 - 0.17% (H.perforatum) & 0.02-0.11 % (H.maculatum). Keywords : hypericin, pseudohypericin, Hypericum, quantitative determination of accumulation