It was investigated if, opipramol (OPI), a tricyclic antidepressant, which has a corresponding double bond for epoxidation, causes any decrease in hepatic glutathione (GSH) level. This was done by administering rats with OPI and then determining their hepatic GSH levels by means of a spectrophotometric method. Both repeated and single low dose (4 mg/kg), and high dose (50 mg/kg) OPI administration produced statistically significant decreases in hepatic GSH level in rats. Keywords : Opipramol, glutathione, liver