¹Department of Pharmaceutical Chemistry, Chandigarh College of Pharmacy, CGC Landran, Mohali, Punjab
²Department of Natural products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar (Mohali)-160062, Punjab, India DOI : 10.29228/jrp.2022.00 Puerarin (PU) nanoparticles were prepared by solvent evaporation method (using PDLG polymer), followed by lyophilization, in order to improve water solubility, systemic adsorption, and dissolution rate. PU preformulation parameters were studied, and optimization study of PU nanoparticles (PU-NPs) was carried out by employing Box–Behnken design (BBD), a response surface methodology. Under optimal conditions PU nanoparticles (PU-NPs) with mean particle size (MPS) 120.6± 0.03 nm and particle size distribution (PDI) 0.22 were prepared. The entrapment efficiency, drug loading and drug content in the PU-NPs were found to be, 90.21%, 14.56% and 98% respectively. The zeta potential at 25℃ was found to be -16.3 mV. PU-NPs were characterized by SEM, FTIR, XRD, TEM, stability, in-vitro release study and cytotoxicity. These results demonstrated that PU-NPs are non-cytotoxic and of smaller particle size than PU. Complete characterization of PU-NPs has shed light on their exceptional characteristics, thus making them a significant asset for subsequent research endeavors. Keywords : Puerarin; PDLG; Cytotoxicity; Solvent evaporation method; Puerarin nanoparticles; Novel formulation