¹Department of Drug Analysis and Pharmaceutical Technology, Faculty of Medicine, University of Prishtina, Prishtina, Kosovo
²Department of Pharmaceutical Technology, Institute of Pharmaceutical Sciences, University of Graz, Graz, Austria
³Department of General Medicine, Faculty of Medicine, University of Prishtina, Prishtina, Kosovo
⁴Technology Research Development Application and Research Centre, Trakya University, Edirne, Türkiye
⁵Department of Pharmacy, Faculty of Medicine, University of Medicine Tirana, Tirana, Albania
⁶Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, North Macedonia DOI : 10.29228/jrp.2022.00 The cytotoxic activity of free and nanoencapsulated essential oil of Lavandula angustifolia (LEO) was evaluated in this study. The aim was to produce different nanoformulations (NF) of LEO to improve the physicochemical properties of NF and the cytotoxic activity of LEO in the DU145 cancer cell line. Essential oil-based liposomes (LEO-Lipoid S100, -Ph 85G, and -Ph 90H) and nanoemulsions (LEO-NE) were prepared by ethanol injection method and high-pressure homogenization, respectively. LEO demonstrates measurable in vitro cytotoxic activity against the DU145 cell line (IC50 75 μg/mL). NE and Ph90H LS significantly enhanced its cytotoxic activity, while LEOLipoid S100 LS and LEO-Ph 85G LS showed no significant difference. LEO-Ph 90H LS and LEO-NE demonstrate stable nanosystems and enhanced cytotoxic potential against the DU-145 cancer cell line, suggesting promising therapeutic benefits for future application. Further studies involving in vivo experiments are necessary to validate and extend these findings. Keywords : Lavandula angustifolia; essential oil; liposome; nanoemulsion; DU145; cancer cell line