¹Department of Chemistry, College of Science, University of Al Mustansiriyah, Baghdad, Iraq
²National Diabetes Center, Mustansiriyah University, Baghdad, Iraq DOI : 10.29228/jrp.2022.00 Biomarkers which identify Diabetic nephropathy (DN) patients could allow early, more efficient intervention and management, decreasing patient morbidity and mortality. This study aimed to estimate the predictive value of cystatin-c protein, and the hormones cortisol and aldosterone in detecting DN in a study including 60 patients with DN under hemodialysis (HD) and other 60 patients with type 2 diabetes mellitus (T2DM) who were recruited from AL-Yarmouk, AL-Kindy and AL- Karama Teaching Hospitals’ dialysis center. Serum levels of cystatin-c, cortisol, and aldosterone were measured for both groups. Serum cystatin-c protein, cortisol and aldosterone hormones levels were significantly higher in patients with DN under HD when it compared with T2DM patients. Receiver operating characteristic (ROC) curve was utilized to test the cut off values of these biomarkers to predict DN results revealed that serum cystatin-c, cortisol and aldosterone levels were significantly higher in patients with DN under HD when it compared with T2DM patients. ROC analysis revealed that the cystatin-C level had the best predictive value in detecting of DN compared with serum cortisol and aldosterone which indicate that cystatin-C could be used to predict DN development and progression in patients with T2DM Keywords : Diabetes mellitus; Diabetic nephropathy; Cystatin-c; Cortisol; Aldosterone