¹Division of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Sanata Dharma University, Campus 3 Paingan Maguwoharjo Depok Sleman, Yogyakarta 55282, Indonesia
²Pharmacy Study Program, Universitas dr. Soebandi, Jalan dr. Soebandi Nomor 99 Jember, Indonesia
³Pharmacy Study Program, STIKES Banyuwangi, Jalan Letkol Istiqlah 109 Banyuwangi, Indonesia
⁴Faculty of Pharmacy, Jember University, Jalan Kalimantan No 37 Jember, Indonesia DOI : 10.29228/jrp.2024.00 Traditional healing culture has long been known in Indonesia, this is proven by the existence of inscriptions that tell about herbal medicine that has been used for generations. As time goes by, the use of plants as medicine has decreased. This is because the inheritance process is only verbal and there is no documentation about these medicinal plants. This encourages research and preservation of plants as medicine, one of which is ethnopharmacy. The implementation of ethnopharmacy in the Osing tribe regarding the use of medicinal plants as anti-diarrhea can provide knowledge about the existence of plants that contain flavonoid compounds which have the potential to act as antidiarrhea. This study aimed at verifying the anti-diarrheal attributes of medicinal plant compounds, Near Infra-Red (NIR) spectroscopy and chemometric techniques were employed. Leveraging the established principle of flavonoid compounds as potent antioxidants capable of stabilizing the digestive tract, we employed flavonoid standards as a reference benchmark for assessing the anti-diarrheal efficacy of these compounds. This approach facilitated a comprehensive evaluation to verify the extent of their anti-diarrheal potential. The results of this research found that the guava leaf plant is widely used by the Osing tribe as an anti-diarrhea. Next, guava leaves were analyzed using Near Infra Red (NIR) spectroscopy and chemometric techniques, and test results were obtained in the form of guava leaf samples from the Osing tribe that contain flavonoids so they can be used for antidiarrheal therapy. Keywords : Ethnopharmacy; antidiarrhea; spectroscopy; chemometrics