Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy Articles in Press
The effect of the ethanolic extract of Laportea decumana (Roxb.) Wedd. on the inflammatory, proliferative and maturation stages of wound healing of an acute injury using a rat model
Dwi Novrianty BUSAERI1,Yulia Yusrini DJABIR2,Rina AGUSTINA3,Gemini ALAM4,Djulfikri MEWAR5,Syaadatun NADIAH6
1Graduate Program, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
2Laboratory of Clinical Pharmacy, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
3Laboratory of Biopharmacy, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
4Drug Discovery and Development Research Center of Hasanuddin University, Makassar, Indonesia
5Pharmacy Department, STIKES Maluku Husada, Maluku, Indonesia
6Faculty of Pharmacy, Muhammadiyah Kalimantan Timur University, Samarinda, Indonesia DOI : 10.29228/jrp.2022.00 Laportea decumana (Roxb.) Wedd. is a plant that is traditionally used for its analgesic, antipyretic, antioxidant, anti-inflammatory, and antibacterial purposes. This study aimed to determine the wound-healing effects of fractionated L. decumana ethanol extract ointment on the inflammatory, proliferation, and maturation phases in a rat model of acute injury. L. decumana leaves were extracted with 70% ethanol and then fractionated with n-hexane with a centrifuge. The polar fraction was used in the animal model. Acute injury was induced in four areas on male rats (n=15), which were assigned to receive either Vaseline, 2% L. decumana extract, 4% L. decumana extract, or Myrhax ointment (control). The wound histological assessments during the inflammatory, proliferation, and maturation phases were conducted on day 1, day 4, and day 9 after injury, respectively. The results show that the wound diameter on Day 9 was significantly lower with 4% L. decumana treatment than with Vaseline and 2% L. decumana treatment and was similar to the results of using Myrhax ointment. Histopathological examination showed that during the inflammatory phase, all wounds exhibited edema, leucocytes, and macrophages; however, during the proliferation phase, 4% L. decumana treatment resulted in significantly more granulation and fibroblasts, as well as thicker collagen and faster reepithelialization during the maturation phase compared to Vaseline-only treatment. In conclusion, 4% L. decumana demonstrated a potent wound-healing effect in the rat acute injury model, especially hastening the proliferation and maturation phases of wound healing. Keywords : Laportea decumana, wound healing, inflammation, proliferation, maturation
Marmara University