¹Department of Pharmacy Kumaun University, 263136, Nainital Bhimtal, Uttarakhand, India DOI : 10.29228/jrp.2022.00 Curcumin, an extract derived from Curcuma longa, boasts a myriad of medicinal applications. In our current research endeavour, we embarked on the formulation of curcumin nanoparticles via the meticulous micro emulsion precursor method, employing the Box-Behnken 32-level design approach. This involved the manipulation of three independent variables, namely, PLGA-Tf-curcumin concentration, stirring speed, and the concentration of the emulsifying agent (span 80). Our investigation revealed that all three independent variables wielded discernible influence over two crucial dependent variables: encapsulation efficiency (EE) and nanoparticle size. It was against this backdrop that we meticulously prepared a total of seventeen formulations. Among this array, formulation F3 emerged as the best to its remarkable EE (99.7±0.2) and a particle size of 214.7 nm. Delving further into our analysis, we scrutinized additional parameters, including drug content (99.7%) and cumulative percentage release (exceeding 99% within a span of 36 hours), both of which yielded highly favourable results. To elucidate the release kinetics, we harnessed the Zero Order, Higuchi, and Korsmeyer-Peppas kinetic models, each revealing an R-squared (R2) value remarkably close to unity. This signifies an exceptionally controlled and diffusion-driven drug release pattern, manifesting in a spherical manner. In this comprehensive assessment, we also scrutinized various other facets, including λmax (wavelength of maximum absorption), particle size distribution, X-ray diffraction, and FTIR analysis. Collectively, these analytical results reinforced the robust authenticity of our study. Keywords : Box-Behnken 32-level design; PLGA-Tf-curcumin concentration; encapsulation efficiency; Higuchi model; X-ray diffraction