¹HKES’s Matoshree Tradevi Rampure Institute of Pharmaceutical Sciences, Kalaburgi, 585105,Karanataka, India
²Department of Pharmacology, Faculty of Pharmacy, Maulana Azad University, Village Bujhawar, Tehsil Luni, Jodhpur 342008, Rajasthan, India
³Department of Pharmaceutics, College of Dentistry and Pharmacy, Buraydah Private Colleges, Buraydah, Al- Qassim 31717, Kingdom of Saudi Arabia
⁴Deccan School of Pharmacy, Darussalam, Hyderabad-500001
⁵Pharmacy College Saifai, UPUMS Saifai, Etawah, 206130, Uttar Pardesh, India
⁶Department of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, City Dammam, Saudi Arabia
⁷Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Quest International University, Ipoh, Perak, Malaysia
⁸Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Maulana Azad University, Village Bujhawar, Tehsil Luni, Jodhpur 342008, Rajashthan, India DOI : 10.29228/jrp.2022.00 The present study was aimed to investigate the potential anti-diabetic effects of Ficus arnottiana Miq. leaves against streptozotocin-induced diabetic model in experimental animals. The leaves of Ficus arnottiana were collected and processed into a powdered form, which was subsequently extracted using methanol as the solvent. The anti-diabetic activity of the resulting extract was evaluated at two oral doses (200 and 400 mg/kg) at two different doses (200 and 400 mg/kg). The parameters assessed in this study included blood glucose levels, C-peptide levels, and serum insulin levels. Furthermore, pancreatic tissue samples were subjected to histopathological analysis. Additionally, in vitro determination of a-amylase and a-glucosidase inhibition by the extract was carried out. The phytochemical screening of the extract revealed the presence of alkaloids, tannins, flavonoids, phenolic compounds, reducing sugars, and saponins. For anti-diabetic activity, the test extract significantly reduced blood glucose levels while increasing both serum C-peptide and serum insulin levels. Histopathological analysis of extract treated group depicted improved islets of Langerhans and an increase in centrally located beta cells. Furthermore, the extract significantly inhibited a-amylase and aglucosidase enzyme in-vitro. Based on these findings, it can be concluded that Ficus arnottiana (Miq) leaves extract exhibits antidiabetic activity. And the anti-diabetic effect of Ficus arnottiana (Miq) at 400 mg/kg was comparable to that of the standard drugs Glibenclamide (in-vivo) and acarbose (in-vitro). Keywords : Anti-diabetic activity; STZ induced diabetes; histopathological evaluation; α-amylase; α-glucosidase; Ficus arnottiana (Miq)