¹Adity Pharmacy College, Surampalem, Andhra Pradesh, India
²Jawaharlal Nehru Technological University Kakinada, Andhra Pradesh, India
³Department of Horticulture, Andhra Pradesh, India DOI : 10.29228/jrp.2022.00 Punica granatum (Pomegranate) is widely accessible and used by natural health professionals for a range of medicinal purposes. The purpose of the current study to use a derivatization method in conjunction with FT-IR, colorimetry, and UV-Visible spectroscopy to determine the total phenolic and flavonoid content of various pomegranate sections. Pomegranate is used to treat ulcers, diarrhoea, Male infertility and so on. Currently, pomegranate is marketed in different novel drug formulations such as nanoparticles for local mucosal drug delivery, niosomes, hydrogels for chronic ınflammation, microemulsion for skin care and analytical techniques using HPLC, GC and GC-MS, LC-MS for carrying out the various quantitative and qualitative analyses are some examples of these developments. In this study, a part of pomegranate was extracted and evaluated by using Microscopical and Macroscopical evaluation, Fluorescence Analysis, FT-IR studies using derivatisation method and finally quantified the phenol and flavonoid content using UVVisible Spectroscopy. Results of the study revealed that the pomegranate extract gives data about the linearity, precision, accuracy of peel and bark ethanolic and aqueous extract gives linearity 1-5μg/ml, Regression 0.998, LOD 0.691μg/ml, LOQ 1.32μg/ml. Through the appropriate selection of Solvents, we successfully developed a stable and effective method for the determination and estimation of phenol and flavonoid content by derivatization and FTIR studies. Method was developed, optimized and validated with its parameters. Keywords : Derivatization method; Flavonoid content; Phenolic content; UV-Visible spectroscopy; FT-IR; Punica granatum (Pomegranate)