¹Department of Analytical Chemistry, Gulhane Faculty of Pharmacy, University of Health Sciences, Ankara, Türkiye
²Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Türkiye DOI : 10.29228/jrp.2022.00 This study aimed to develop an electrochemical method for the electrochemical determination of bufexamac (BUF), an important anti-inflammatory drug. For this purpose, the electrochemical properties of BUF were elucidated using a glassy carbon electrode (GCE) and cyclic voltammetry. The electrochemical performance of BUF was examined by the differential pulse voltammetry (DPV) method. It was observed that the response of GCE to DBT was linear in the concentration range of 6.0 × 10–6 M and 1.0 × 10–4 M. The limit of detection (LOD) was found to be 2.64 × 10−8 M; The limit of quantification (LOQ) was calculated as 8.79 × 10−8 M. The dexketoprofen tromethamine (DEX), diclofenac (DIC), and ibuprofen (IBU) were used as model active compounds to illustrate the oxidation mechanism of BUF. A simple, rapid, and sensitive electrochemical method for quantitatively determining BUF in a commercial sample of human serum was developed and validated. The calibration curve was linear in the 8.0 × 10–6 – 1.0 × 10–4 M in the biological media. The LOD for the commercial sample of human serum was set at 1.59 × 10−7 M. The electrochemical response of BUF in the presence of interferent was evaluated. The data obtained from the study showed that the developed method can be used in sensitive and selective BUF determination. Keywords : Bufexamac; determination; electrochemical sensor; glassy carbon electrode; validation