¹Anjuman-I-Islam's-Kalsekar Technical Campus, School of Pharmacy, New Panvel-410206, Maharashtra, affiliated to Mumbai University, Mumbai, India DOI : 10.29228/jrp.831 Habbe Sara is an herbal formulation used in the Unani system of medicine to treat convulsions in adults and children. It is believed to strengthen the brain and nervous system, reducing the frequency of fits. In this study, the effects of various concentrations of Habbe Sara ranging from 50 to 400 ppm on in zebrafish were examined. The study found that increasing concentrations of Habbe Sara resulted in higher mortality rates with an LC50 value of 251.18 ppm. In addition, its higher concentrations showed delayed growth, decreased in heartbeat rate, reduced hatchability, and various developmental defects in the treated embryos. Therefore, caution should be exercised when administering high doses of Habbe Sara to pregnant women. Keywords : Teratogenic effect; Habbe Sara; developmental defect ; zebrafish; toxicity