Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2024 , Vol 28 , Issue 5
Comparison of the effect of UVB application on the antioxidant properties of different mushroom species with vitamins D2 and D3
Aybike CEBECİ1,Turgut TAŞKIN2,Şule AKTAÇ3,Muhammet Emin ÇAM4,Rıfat Sami SANCI5,Fatma Esra GÜNEŞ6
1Department of Nutrition and Dietetics, Institute of Health Sciences, Marmara University, İstanbul, Turkey
2Department of Pharmacognosy, Faculty of Pharmacy, Marmara University, İstanbul, Turkey
3Department of Nutrition and Dietetics, Faculty of Health Sciences, Marmara University, İstanbul, Turkey
4Department of Pharmacology, Faculty of Pharmacy, İstanbul Kent University, İstanbul, Turkey
5Elit Engineering, İstanbul, Turkey
6Department of Nutrition and Dietetics, Faculty of Health Sciences, İstanbul Medeniyet University, İstanbul, Turkey DOI : 10.29228/jrp.829 This study aimed to determine the effect of UVB application on different mushrooms and their antioxidant properties and compare their properties with pure vitamins D2 and D3. In the study, as without UVB, with medium UVB and with high UVB, respectively; 3 ethanol extracts each from White button (E1, E2 and E3), Shiitake (E4, E5 and E6) and King oyster mushroom (E7, E8 and E9) were prepared. The antioxidant activities of the samples were compared with pure vitamin D2 and D3 solutions using DPPH, FRAP, and CUPRAC methods. The results show that vitamins D2 (0.277 mMFeSO4/mg extract) and D3 (0.380 mMFeSO4/mg extract) had the highest FRAP values. In mushroom species, it was determined that E3 (0.216 mMFeSO4/mg extract) and E9 (0.273 mMFeSO4/mg extract) mushroom extracts showed higher activity. Similarly, it was detected that vitamins D2 (0.609 mMrolox equivalent/mg extract) and D3 (1.149 mMrolox equivalent/mg extract) had the highest CUPRAC values. In mushroom species, E3 (0.324 mMrolox equivalent/mg extract) and E9 (0.292 mMrolox equivalent/mg extract) extracts had the highest CUPRAC values. According to the DPPH experiment, E6 (IC50:0.386 mg/ml) and E9 (IC50:0.411mg/ml) extracts showed stronger free radical scavenging activity compared to other extracts, while vitamin D forms showed lower activity than mushroom extracts. Ultimately, it was concluded that the antioxidant properties of mushrooms were positively influenced by the application of UVB. Keywords : Mushrooms; antioxidant activity; ultraviolet rays; ergocalciferol; cholecalciferol
Marmara University