¹Laboratoire de Biotechnologie, Ecole Nationale Supérieure de Biotechnologie, Constantine, Algeria
²Centre de Recherche en Biotechnologie, Ali Mendjli Nouvelle Ville UV 03, BP E73 Constantine, Algeria DOI : 10.29228/jrp.799 This study evaluated the antioxidant, antidiabetic, anticholinesterase, anti-inflammatory, and antimicrobial effects of Algerian Cleome amblyocarpa through its chloroform fraction. Furthermore, phenolic, flavonoids and volatile compounds have been studied. The results indicated high phenols and flavonoids contents, and the gas chromatographymass spectrometry (GC-MS) analysis revealed the presence of various compounds, mainly 2- Tetracosane, Palmitic acid, 2- Aminothiazole, and Linolenic acid. Previous research supports the potential antidiabetic effects of palmitic acid and linolenic acid, while tetracosane has been studied for its anticholinesterase activity, validating the observed biological activities in our research. The fraction has demonstrated a substantial anti-inflammatory activity similar to the reference, antimicrobial actions against Escherichia coli, Klebsiella pneumonia, Bacillus cereus, Pseudomonas aeruginosa, and Candida albicans, as well as an antioxidant potential, and significant inhibition of key enzymes: alpha-amylase, alpha-glucosidase, acetyl, and butyrylcholinesterase (AChE and BChE), with interesting IC50 values. Our result found that Cléome amblyocarpa is a rich source of value-enhancing biocompounds, and it presents interesting antioxidant, antidiabetic, and anticholinesterase properties, as well as substantial anti-inflammatory and antimicrobial activities. These findings can be exploited in developing new formulations that may be useful in treating a variety of situations. Keywords : anti-enzymatic; anti-inflammatory; antimicrobial; antioxidant; Cléome amblyocarpa Barr. & Murb. ; GC–MS