¹Biology Department, Faculty of Mathematics and Natural Sciences, Andalas University, Padang, Indonesia DOI : 10.29228/jrp.808 The wound is a local response of a tissue caused by contact with a heat source, sharp object, or microbes. Some pathogens have developed resistance to several topical antibiotics and cause adverse side effects. Therefore, the search for natural anti-inflammatory drugs that are effective but have few side effects is urgently needed. One of the natural ingredients that have the potential as anti-inflammatory is Black Soldier Fly (BSF) prepupa oil. This study aims to reveal the potential of BSF prepupa oil in accelerating burn wound healing and explain the content of chemical compounds in BSF prepupa oil as an anti-inflammatory using gas chromatography-mass spectrophotometry (GCMS) analysis. This study used 48 male BALB/c mice with four treatment groups, namely control group (without any treatment), 0,1 g Bioplacenton® treated group three times a day, 20 μL BSF prepupa oil-treated groups with once a day or two times a day applications. Burn wound conditioning was done by administering 25% phenol for 30 seconds, after which it was left for one day and then treated. Furthermore, changes in wound morphology, a decrease in the wound area, and leukocyte count were observed. The results showed that administering 20 μL BSF prepupa oil twice a day had a considerable anti-inflammatory effect on burn wound healing, as indicated by morphological changes in scab detachment and a significant decrease in wound area on day 7. However, it did not affect the number of leukocyte components in mice. In addition, GC-MS analysis revealed several anti-inflammatory solid compounds, namely acetic acid, ethenyl benzene, lauric acid, propionic acid, stearic acid, palmitic acid, and linoleic acid. Keywords : Anti-inflammatory; black soldier fly prepupa; burns; hematology; linoleic acid