¹Physiology, Biochemistry & Pharmacology Department, College of Veterinary Medicine, University of Baghdad, Iraq DOI : 10.29228/jrp.794 Tramadol and remifentanil, which is an opioid analgesic are known to have a local anesthetic effect and the present study aimed to evaluate the local anesthetic efficacy of them on twenty-five adult male rabbits Oryctolagus cuniculus (aged between 10-12 months and weighing 1.5±0.08g) who were divided into five groups received the following treatment subcutaneously for 5 days; G1: 5% Tramadol 15 mg/kg BW; G2: 2% Remifentanil 2 mg/kg BW; G3: 2% Lidocaine 4 mg/kg BW; G4: 2% Remifentanil with epinephrine 0.00l mg/kg BW; and G5:2% Remifentanil with Carbopol gel 2 mg/kg BW. Baseline, pre-anesthetic, and post-anesthetic physiological parameters, including temperature, respiratory rate, and heart rate, were meticulously recorded for all groups following local administration of lidocaine, tramadol, and remifentanil. The results showed that tramadol caused a significant decrease in the temperature while other groups showed non-significant effect, results also revealed that pain evaluation that performed according to Grimace Scale scores and by the behavioral analysis of pain assessment elucidated that Lidocaine treatment group showed a statistically significant lower pain response compared to the Tramadol and Remifentanil groups and further significant improvement in pain were observed with the addition of epinephrine or Carbopol gel to Remifentanil. Both combinations resulted in a statistically significant decrease in pain parameters for all measured features comparing with Tramadol and Remifentanil groups and these results leads to conclude that tramadol and remifentanil showed a comparable anaesthetic effect that improved significantly with epinephrine or carbopol gel as local anaesthetics that may surpass lidocaine local anaesthetic effect significantly. Keywords : lidocaine; remifentanil; local anesthetics; tramadol; rabbits; adjuvants