We have tested hemoglobin A₁C In 10 newborns. Methods which were used in this study were used in this study were ion exchange and affinity- chromatography for the separation and quantitation of glycosylated hemoglobin from whole blood. The amount of hemoglobin A₁C in newborns showed a significant difference depending on the methods used. Ion exchange chromatography result showed that the amount of HbA₁C C of same newborns increased 3-4 times more with the affinity chromatography results. Keywords :