Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2024 , Vol 28 , Issue 2
Supercritical carbon dioxide extraction optimization of Brassica oleracea var. capitata f. rubra leaf extracts for cholinesterase and tyrosinase inhibitory activity
Halil KOYU1
1Department of Pharmaceutical Botany, Faculty of Pharmacy, Izmir Katip Celebi University, Cigli 35620 Izmir, Türkiye DOI : 10.29228/jrp.710 Supercritical carbondioxide extraction has been an advanced system as the regulation of extraction parameters enables the control of the solvating power, resulting in a more selective process. While the efficient use of natural resources is critical for sustainability goals, the discovery of feasible plant sources for medicinal purposes is a valuable research objective. In this study, supercritical carbondioxide extraction of red cabbage (Brassica oleracea var. capitata f. rubra) was investigated using response surface methodology. A statistical design was used for the evaluation of the effect of extraction parameters as temperature (40, 60, 80°C), pressure (120, 160, 200 bar) and co-solvent ratio (0, 6, 12%). Spectrophotometric and UPLC-DAD, ESI-MS/MS analysis were performed to investigate total phenol, flavonoid, anthocyanin and dominant anthocyanin contents. The optimization models were determined as significant for all bioactive content analysis and enzyme inhibitory activities. 40°C, 160 bar, 12% co-solvent conditions were determined with highest total flavonoid content (47.11 mg QE/g). None of the supercritical carbondioxide extracts were able to extract red cabbage anthocyanins within the investigated parameter ranges. 60°C, 120 bar, 12% co-solvent extract showed the strongest tyrosinase inhibitory activity (IC50: 1.71 mg/ml). Cholinesterase inhibitory activity of supercritical carbon dioxide extracts was found to be comparable to that of solvent extracts considering the tested concentrations. Optimum supercritical carbondioxide extract can be considered as a potential source with tyrosinase inhibitory activity, while identification of active molecules, especially flavonoids, can be targeted in further isolation studies. Keywords : Acetylcholinesterase; Brassica oleracea var. capitata f. rubra; butyrylcholinesterase; extraction; LCMS/ MS; red cabbage; supercritical carbondioxide
Marmara University