Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2024 , Vol 28 , Issue 2
Antibacterial activity and cytocompatibility evaluation of cefpodoxime proxetil loaded eudragit rs 100 nanoparticles
Irfan AKARTAS1,Aysegul ATES2,Rengin REIS3
1State Laboratory, Ministry of Health, Turkish Republic of Northern Cyprus, TR-10, Mersin, Turkey
2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, Izmir, Turkey
3Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Acıbadem Mehmet Ali Aydınlar University, Atasehir, Istanbul, Turkey
DOI : 10.29228/jrp.706 The main purpose of this study is to design Cefpodoxime proxetil (CFP) loaded Eudragit RS 100 (ERS) nanoparticles to provide prolonged drug release, the enhanced oral efficacy of the drug at a lower oral dose. CFP-loaded nanoparticles are prepared by the single-emulsion solvent evaporation method with different drug:polymer ratios, F1 (CFP:ERS=1:1) and F2 (CFP:ERS=1:2). Physicochemical characterization, antibacterial activity, cytotoxicity profiles of nanoparticles are examined. F1 and F2 nanoparticles have a particle size of 236.70±3.05 and 258.87±11.32 nm, spherical shape, and a porous surface under SEM. The zeta potential values of F1 and F2 nanoparticles were 22.13±1.01 and 25.80±2.01 mV, and the encapsulation efficiencies were 34.37±3.71% and 55.78±3.52%, respectively. Since F2 nanoparticles have a smaller particle size, higher zeta potential, and encapsulation efficiency, this formulation was chosen as superior, and the subsequent experiments were continued with this formulation. The antibacterial activity of F2 nanoparticles against E.coli was found to be 2 times more effective than CFP. Cytotoxicity studies revealed that the cell viabilities in BEAS-2B and HepG2 cells were higher with the exposure to CFP nanoparticles (200 μg/ml) compared to CFP (100 μg/ml) alone. CFP nanoparticles have excellent efficacy and a cytocompatible profile in inhibiting the growth of bacteria compared to free CFP. Keywords : Cefpodoxime; eudragit; nanoparticles; oral application; antibacterial; time kill; cytotoxicity
Marmara University