¹Department of Chemistry, GLA University, Mathura, UP-281406, India
²Department of Chemistry, University of Lucknow, Lucknow UP-226007, India
³Department of Chemistry, Kalindi College, University of Delhi, Delhi, India DOI : 10.29228/jrp.699 The importance of developing chiral chromatographic methods to monitor and ensure the purity of enantiomers is underscored by the distinct biological properties exhibited by the different forms of ketoprofen. We employed a thin-layer chromatographic approach to separate ketoprofen from commercially available tablet formulations. Our method utilized frovatriptan as the chiral selector, which was incorporated into the stationary phase through a silica gel slurry. The mobile phase consisted of a combination of acetonitrile, methanol, and triethylamine in a ratio of 6:2:2, with a pH of 9. Frovatriptan also served as the chiral selector in the stationary phase, maintaining a pH of 5. The detection and quantitation limits for the ketoprofen enantiomers were determined to be 8.4 μg spot-1 and 25.2 μg spot-1, respectively. Notably, this study marks the first-ever application of frovatriptan as a chiral selector in thinlayer chromatography to resolve ketoprofen enantiomers. Keywords : Enantioresolution; pharmaceuticals; ketoprofen; NSAIDs; Frovatriptan; Chiral chromatography; method validation