Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Journal of Research in Pharmacy
2024 , Vol 28 , Issue 1
In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage
1Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ankara University, Yenimahalle 06560 Ankara, Turkey
DOI :
10.29228/jrp.682
Salmonella is an important agent that causes foodborne gastrointestinal infections. Salmonella enterica
serovar Enteritidis, serovar Typhimurium, and serovar Infantis are the main pathogenic agents of several enteric
infections worldwide. Due to the emergence of multidrug resistant Salmonella strains, there is a need for new
administration strategies alternative to antibiotics, such as bacteriophages, for effective control of them. The main
objectives of this study were to isolate and characterize Salmonella spp. bacteriophage and compare its activity to
commercial phage product and assess the effect of bacteriophages in vitro during 24-h. Because of the study, it was
found that the newly isolated and named vB_SiM_12 phage could lyse 100% of the strains tested. Bacterial growth with
dilutions of vB_SiM_12 phage (MOI ranging from 0.001 to 10) was measured during 24-h. The optic density values of
the samples with added bacteriophage remained lower than the control sample during the 24-h incubation period. The
results show that using phages may offer a promising alternative to combat biological control agents against Salmonella
infection.
Keywords :
Bacteriophage; bacteriolytic activity, invitro turbidity test; Salmonella spp..