¹Doctoral Program Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
²Natural Product Medicine Research and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
³Research Center on Global Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
⁴Magister Program of Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
⁵Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia DOI : 10.29228/jrp.681 Malaria is one of the tropical diseases that become a health problem worldwide. The elimination of this disease faced various burdens, leading to the need for new antimalarial drugs. Based on the previously reported antimalarial activity of Artocarpus genus, Artocarpus sericicarpus has been selected as one of the candidates which are potential to be explored as a source of antimalarial substances. This study aimed to obtain active antimalarial fractions from A. sericicarpus leaves. Extraction of A. sericicarpus leaves was conducted by ultrasonic-assisted extraction using nhexane, dichloromethane, and methanol as solvents. All extracts were tested for their antimalarial activity by Lactate Dehydrogenase (LDH) assay. The most active extract was fractionated by Vacuum Liquid Chromatography (VLC) and fractions were further tested by LDH assay as well. Cytotoxicity test conducted by resazurin assay on several cell lines to determine the active and nontoxic fractions which have the potential to be further purified and identified the active substances. The n-hexane, dichloromethane, and methanol extract showed antimalarial activity with IC50 values of 23.96±0.06 μg/mL, 2.72±0.08 μg/mL, and 23.39±0.05 μg/mL, respectively. Dichloromethane extract was chosen for further separation due to its highest antimalarial activity. The separation was obtained in nine fractions. Fractions 2-9 had an IC50 value of less than 10 μg/mL, indicating an active antimalarial substance. Meanwhile, fraction 1 has moderate antimalarial activity. Cytotoxicity test results considered all fractions to be non-toxic. The TLC profile of dichloromethane extract identified polyphenolic compounds, suggesting the active polyphenolic compounds can be further isolated from the active fractions (F2-F9). Keywords : Artocarpus sericicarpus; cytotoxicity; extracts; fraction; malaria