¹Department of Clinical Laboratory Sciences, Faculty of Pharmacy, Mosul University, Mosul, Iraq DOI : 10.29228/jrp.543 Otitis externa refers to an acute or sub-acute inflammatory condition affecting the skin of external ear and its uricle. Otitis externa is a common disease in all regions especially in tropical and subtropical areas and is usually caused by bacterial or fungal infections. This study aimed to study the pattern of different antifungal drugs activity by disc diffusion method on fungi isolated from patients having otitis externa attended ENT clinics of different age group of both sexes over one-year period in Mosul city/Iraq. One hundred patients identified clinically as having otitis externa and 100 control group were included. Skin scrap and swab of discharge were taken from each patient. The clinical specimens were examined macroscopically and microscopically to identify the species and genus of fungi involved in otitis externa. Among the 100 patients included, 64 (64%) showed fungal elements in their clinical specimens, 36 (56%) were male and 28 (44%) were females. The isolated fungi of genus Aspegillus were 38 (59%); Aspergillus niger 24 (37%), Aspergillus flavus 14 (22%). Isolates of the genus Candida accounted 22 (34%); 20 (31%) were Candida albicans and 2 (3%) were Candida glarata. Penicillum spp. was identified in 3 (5%), and Rhizopus spp. was identified in 1 (2%) sample. However, coinfection with Aspergillus niger and Candida albicans was identified in 2 (3%). On the other hand, Candida albicans was identified in 8 (8%) of control group. Seasonal variation was found in this study, the highest number of otitis externa cases was found in June, July and August. In this study, males were found more affected by otitis externa than females. Aspergillus niger and Candida albicans were the most commonly encountered species. Seasonal variation was reported with higher percentage in summer months. Voriconazole and ketoconazole have had the higher activity against fungi isolated in this study than other antifungal agents. Keywords : Candida, Fungi; Otitis externa; Sensitivity test