Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 6
Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell
1Ataturk University, Faculty of Veterinary Medicine, Department of Microbiology, 25240 Erzurum, Turkey.
2Ataturk University, Ataturk University Vaccine Application, and Development Center, 25240 Erzurum, Turkey.
3Ataturk University, Faculty of Medicine, Department of Medical Microbiology, 25240 Erzurum, Turkey.
4Seyh Edebali University, Faculty of Medicine, Department of Medical Pharmacology, 11000 Bilecik, Turkey.
DOI : 10.29228/jrp.516 Liver abscesses are most commonly attributed to Escherichia coli and Klebsiella pneumoniae, but because Streptococcus mitis is a commensal bacterium with low virulence activity, it is overlooked by its antimicrobial resistance and virulence factors. In this study, we tried to evaluate the synergistic effect of boron compounds and the effect on biofilm in the infection model we created with Streptococcus mitis NCTC 12261 on the HepG2 liver cell line. Minimal inhibitory concentration (MIC) values were determined on Streptococcus mitis at doses of 31.25 μg/ml of Sodium Perborate Metahydrate (SPM), Zinc Borate (ZB) 62.5 μg/ml and Etidote 125 μg/ml. Etidote+SPM, Etidote+ZB, and ZB+SPM combinations showed the most effective fractional inhibitor concentration value synergistic effect at minimum doses of 32 μg/ml+32 μg/ml in all combinations, while it showed an effect on biofilm at doses of 1028 μg/ml+ 1028 μg/ml HepG2 Cell culture. Keywords : Biofilm, Boron Compounds, Liver Cell, Synergistic Effect, Cytotoxic effect
Marmara University