All approved gene-based biopharmaceuticals were all prepared with the viral vector. These viral vectors have been critical in realizing the therapeutic potential of genebased biopharmaceuticals [7-9]. Unfortunately, undesired viral vector-mediated immune responses remain a major safety concern. The use of non-viral vectors increases because of the potential of viral vector titer and immunogenicity [8, 9]. In contrast to viral gene therapy systems, non-viral gene therapy systems utilize nanometer-sized synthetic and biological-derived materials as basic vector building blocks for gene delivery vector construction and harnesses bio-physicochemical interactions between nanomaterial and nucleic acid cargos to achieve cargo encapsulation and cellular delivery [10].

Polymers have been extensively utilized for biomedical drug delivery applications. Biodegradable drug delivery systems have great biocompatible and biodegradable properties. Additionally, they can easily be manipulated for expected modifications [1, 7, 10]. Gelatin and cellulose derivative polymers have been widely used to prepare gene-delivery vectors by their low antigenicity and high biocompatibility. Cationic polymers are generally preferred to form nucleic acid-polymer complexes, due to the lack of positively-charged motifs. Relevant transfection results indicated that cationic polymers with a high molecular weight show better gene complexing ability, cell uptake, and transfection efficiency [1, 4, 5].

In order to increase the effectiveness of non-viral drug delivery systems, they need to be overcome extracellular and intracellular barriers. The biologically active substance may degrade due to heat and sheer stress during formulation. Also, the reticuloendothelial system are responsible for the clearance of biologics in blood circulation. Additionally, a number of enzymes rapidly inactivate them after systemic application. Drug delivery systems also help the transition from the plasma membrane. They can also trigger an inflammatory response, since they do not provide a specific recognition [1, 10-12].

Drug carrier systems are called by different names according to the auxiliary materials and methods used. Liposomes are biologic membrane-like sacs in sphere form consist of lipidic barriers. The advantages of liposomes include effectiveness at small doses, extended dosing interval, and ideal transport for active substances with a short half-life [6]. Dendrimers are nanostructures which have functional groups on their surface. Micellar structure is obtained with the use of appropriate surfactant group. The micellar structure is obtained with the use of an appropriate surfactant group. Carbon nanotubes, DOT matrices, and gold particles are the other carrier systems used for biologicals. Hybrid systems will be developed in the near future to combine the advantageous parts of both the individual systems. Moreover, the design of smart vectors capable of releasing their loaded protein payloads in a timely and spatially-controlled manner is crucial for the development of next-generation biological delivery vectors. Keywords : Biologicals, viral drug delivery systems, non-viral drug delivery systems