¹Department of Chemistry, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, Tangerang Selatan, 15412, Indonesia
²Department of Agribusiness, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, Tangerang Selatan, 15412, Indonesia DOI : 10.29228/jrp.483 Indonesian honey contains active compounds that have the potential as antioxidant and anticancer, especially as anticancer of the larynx through inhibition of HEp-2 cells. This study aims to determine the active compounds in longan honey and proposes the mechanism of action in inhibiting HEp-2 cells. The sample was used in the form of longan obtained from honey bee breeders in Central Java. Honey samples were extracted using methanol, and then liquid-liquid partitioning was carried out successively using n-hexane and ethyl acetate. Isolation and characterization of longan honey samples using FTIR and LC-MS/MS showed the presence of the following compounds: Xanthoxol glycosides, Santonin, Octadecanamide, Indole-3 carboxylaldehyde, 3,4-dimethoxycinnamic acid, Dimethyl esculetin, Tryptophan, O-acetyl-L- serine, D-glucose-6-phosphate, Feruloiltyramine, Lauryl diethanolamide, Taurine, 6-mercaptopurine, 3-(2,4-dichlorophenyl)-4-phenylcoumarin, 3',4'-dimethoxy-3-hydroxy-6- methylflavone and D-1-((3-carboxypropyl)amino)-1-deoxyfructose. The compounds in longan honey against HEp-2 cells is quercetin, 3,6-dimethoxycinnamic acid, phenyl coumarin, dimethyl esculetin, santonin, 6-mercaptopurine, and feruloyltyramine. The proposed mechanism of active compound in honey to inhibit HEp-2 cells in several ways including via caspase pathway and purine synthesis and other relevant mechanisms. However, this assumption needs to be tested further to obtain more precise information regarding the mechanism of inhibition of HEp-2 cells. Keywords : HEp-2 Cells; inhibition; longan honey; LC-MS/MS; FTIR