Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 5
Electroanalytical investigation and voltametric quantification of emtricitabine in the biological sample using boron-doped diamond and glassy carbon electrodes
1Department of Analytical Chemistry, Faculty of Pharmacy, Adıyaman University, Adıyaman, Türkiye
2Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Türkiye
DOI : 10.29228/jrp.481 Emtricitabine (EMT) was electrochemically investigated in detail by using boron-doped diamond electrode (BDDE) and glassy carbon electrode (GCE). The measurements were performed via cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. EMT exhibited one irreversible oxidation peak on the BDDE by CV, with dependence of the pH values. It also showed one irreversible oxidation peak on the GCE via CV from pH 0.3 until pH 6.0. Two irreversible peaks were observed at pH 6.0 with phosphate buffer (PB). The aim of this work is to develop a system able to detect EMT in synthetic serum samples using the aforementioned electrochemical techniques. All required validation parameters were examined and computed. The developed sensors were successfully used to analyze EMT in synthetic serum samples. The standard addition technique was applied in the recovery studies with both electrodes. At 1.0x10-3 M EMT, the highest voltametric peak response was achieved in Britton-Robinson Buffer (BRB) at pH 11.0 by BDEE, and 0.5 M H2SO4 by GCE. Good linearity was observed for a concentration range between 1x10-3 to 4x10-5 M and 1x10-3 to 6x10-5 M, with limits of detection of 1.66 μM and 2.91 μM, for BDDE and GCE, respectively. Keywords : Emtricitabine; boron-doped diamond electrode; glassy carbon electrode; drug assay; differential pulse voltammetry
Marmara University