¹Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University-Cerrahpasa, 34500 Istanbul, Turkey
²Department of Pharmaceutical Technology, Faculty of Pharmacy, Bezmialem Vakif University, Fatih, Istanbul, Turkey DOI : 10.29228/jrp.466 Retinyl palmitate (RP), also known as vitamin A palmitate, is a well-known anti-aging ingredient that promotes collagen production, enhances skin flexibility, and decreases the view of fine lines and wrinkles. Squalene, a natural lipid found in the skin, is also known to have anti-aging properties, including moisturizing and protecting the skin from environmental stressors. In this study, we demonstrate the encapsulation of RP and squalene within niosomes, and evaluate the in-vitro release and skin permeation of these active ingredients. The niosomes were prepared using a thin-film hydration method and characterized for their particle size, polydispersity index, zeta potential, and encapsulation efficiency. The encapsulation efficiencies of optimized niosome formulation for RP and Squalene were determined to be 83.5%±2.55% and 91.2%±5.12%, respectively. Narrow size distribution was accomplished with a particle size of 168.4 ± 8.2 nm. Cumulative release of 82.67%±5.23% for RP and 87.12%±5.65% for Squalene was acquired after 24 hours of in-vitro release study in sink conditions. Ex-vivo permeation studies indicated that niosomes had a much higher retention rate for RP and Squalene in both the Stratum Corneum (SC) and viable epidermis/dermis layers when compared to the commercial product. Stability studies showed that the optimized niosomes were convenient for keeping at 4°C for at least 90 days. The outcomes show that niosomes are effective in delivering RP and squalene to the skin and can provide a promising alternative to traditional anti-aging formulations. Keywords : Retinyl palmitate; Squalene; Niosomes; Skin permeation; Epidermal targeting