Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 5
9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation
Babanna YELUGUDARI1,Nageshwar MESRAM1,Pratap Reddy KARNATI1
1Department of Zoology, Faculty of Neurobiology, Osmania University, Hyderabad, India DOI : 10.29228/jrp.458 Pithecellobium dulce (Roxb.) Benth. has been used as a folk medicine due to its wide range of pharmacological applications. In light of the lack of significant studies examining the anti-inflammatory potential of seeds and their phytochemical constituents, we investigated the molecular basis behind the therapeutic efficacy of methanolic extract both in-vitro and in-vivo. The phytochemical investigation of methanolic seed extract using GC-MS analysis revealed the presence of 30 molecules of diverse classes. Further solvent fractionation followed by RP-HPLC separation demonstrated the presence of 9-Hexadecenoic acid and 8,11,14 (Z, Z, Z)-Eicosatrienoic acid and were further structurally characterized by 1H-NMR and HRMS. 9-Hexadecenoic acid-rich HPLC fraction exhibited the highest % inhibition of albumin denaturation and % reduction of IL-8 and IL-6 in HaCaT cells as well as NO and PGE-2 production in RAW267.4 cells among other test samples. In-vivo anti-inflammatory findings revealed that 9-Hexadecenoic acidrich fraction potentially reduced rat paw edema by 84.62% at 150 μg/mL, which is superior to the diclofenac. The results of the present study revealed that the 9-hexadecenoic acid-rich fraction of methanolic seed extract of P. dulce displayed potential anti-inflammatory activity both in-vitro and in-vivo, which suggests an efficient alternative in treating a variety of ailments related to inflammation and pain. Keywords : Pithecellobium dulce; Methanolic seed extract; Anti-inflammatory activity; 9-hexadecenoic acid; GC-MS; HPLC
Marmara University