Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Journal of Research in Pharmacy
2023 , Vol 27 , Issue 4
Anti-inflammatory effects of velvet bean (Mucuna pruriens L. (DC.), Fabaceae) leaf ethanolic extract against carrageenan in male mice
1Biology Department, Faculty of Mathematics and Natural Sciences, Andalas University, Padang, Indonesia
DOI :
10.29228/jrp.438
Inflammation is an immune response that could lead to serious health problems. Some steroids and nonsteroidal
drugs are capable of relieving inflammatory reactions but may promote various detrimental side effects.
Therefore, the search for effective but less side effects of natural antiinflammatory drugs are urgently needed. One of
the medicinal plants that may have the potential as an anti-inflammatory is velvet beans (Mucuna pruriens L. (DC.)).
This study aimed to investigate the effectiveness of velvet bean leaf ethanolic extract against inflammation in mice as
an animal model and to elucidate the phytochemical constituents of velvet bean leaf extract regarding their antiinflammatory
properties by deploying gas chromatography-mass spectrophotometry (GCMS) analysis. This study used
40 male BALB/c mice with 4 treatment groups, namely negative control (Na-CMC 1%), positive control (diclofenac
sodium 4.5 mg/kg BW), velvet bean leaf extract 200 mg/kg BW, and 400 mg/kg BW, respectively. The extract or
diclofenac sodium was given orally to the mice 30 minutes upon intraplantar injection of carrageenan 1%. Subsequently,
the volume of paw edema, area under curve (AUC) values of edema, and percent of anti-inflammatory power as well
as leukocyte counts were determined. The results demonstrated that ethanolic extract of velvet bean leaf, particularly
at the dose of 400 mg/kg BW exerted a substantial anti-inflammatory effect against carrageenan as indicated by the
lowest edema volume, lowest AUC value, the highest anti-inflammatory power, and stronger suppression on leukocyte
counts. In addition, the GC-MS analysis revealed some potent anti-inflammatory compounds namely hexadecanoic
acid, geranylgeraniol, geraniol, 3-aminobenzamide, octadecanoic acid, and 4-hydroxycinnamic acid.
Keywords :
Carrageenan; edema; hexadecanoic acid; inflammation; velvet bean