¹Division of Pharmaceutical Biology, Faculty of Pharmacy, Sanata Dharma University, Campus III, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282 Indonesia
²Faculty of Humanities and Health, Department of Pharmacy, Mulia University, Jl. Letjend. Zaini Azhar Maulani No. 9, Balikpapan, East Kalimantan 75123 Indonesia
³Division of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Sanata Dharma University, Campus III, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282 Indonesia DOI : 10.29228/jrp.428 Shallot skin has potential as a medicinal raw material because it contains many quercetin compounds. It is necessary to conduct a study on the optimization of extraction methods and the extracts standardization. The purpose of this study is the standardization of simplicia, optimization of extraction methods and standardization of shallot skin purified extract as standardized on quercetin. This study is aimed to support quality control of simplicia and shallot skin extract as a source of quercetin. The methods of the study include the collection of raw materials, preparation of simplicia, standardization of simplicia, and optimization of extraction methods with Response Surface Methodology (RSM) using Box-Behnken Design (BBD), standardization of standardized quercetin purified extracts. Based on the results obtained, shallot skin simplicia has a moisture content of 7.98 and a total ash content of 9.91%. Based on the analysis, the optimum point of the solvent concentration factor, time, and the solvent ratio on the yield of the purified extract were respectively 60.42%, 1.29 hours, and 24.45 mL/g. The yield average obtained was 5.16 ± 0.1125 % with a content of 5.947 ppm (0.005497 mg/g). Keywords : purified extract; quercetin; shallot skin; maceration; Response Surface Methodology (RSM)