¹Department of Pharmacognosy, Faculty of Pharmacy, İzmir Katip Celebi University, Izmir, Turkey
²Department of Pharmacognosy, Faculty of Pharmacy, Ege University, Izmir, Turkey
³Department of Medical Biology, Faculty of Medicine, Bakırcay University, Izmir, Turkey
⁴Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey DOI : 10.29228/jrp.413 The aim of the study is to examine the cytotoxic, apoptotic effects and gene expressions of Asphodelus aestivus water extract (ASP) and silver nanoparticles (AgNPS) on decided cancer lines. Breast cancer cell lines MCF-7 and MDA- 231; melanoma cancer lines MEWO and CHL-1; fibroblast cancer lines WI-38 and HEL 299 were selected for biological activities. xCELLigence system was used for cytotoxicity. Annexin V-EG FP Apoptosis detection kit was used for apoptosis and gene expressions were assessed by real time online RT-PCR by using cancer cell lines Qiagen kits. AgNPS showed significant cytotoxicity in all cell lines. The most prominent apoptosis was determined in MCF-7 cell line for AgNPS. It has been observed that the most important progress in gene expression, the suppression capacity of MUC1-C, a breast cancer- associated oncoprotein, is greatly increased by nanoparticle formation. In addition, cells that were not affected at all in MDA-MB-231 breast cancer oncoprotein started to suppress with nanoparticles. In conclusion, it was determined that silver nanoparticles increased the effect especially in breast cancer cell lines and could be considered for use. Keywords : Green synthesis; apoptosis; cancer; melanoma; cancer cell lines; silver nanoparticles