Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 3
The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L.
Selma SEZEN1,Muhammed Sait ERTUĞRUL2,Cemil BAYRAM3,Mustafa ÖZKARACA4,Taha Yasin KOÇ5,Abdussamed Yasin DEMİR6,Medine GÜLLÜCE7,Ahmet HACIMÜFTÜOĞLU8
1Department of Medical Pharmacology, Faculty of Medicine, Agri Ibrahim Cecen University, Agri, Turkey
2Hemp Research Institute, Ondokuz Mayıs University, Samsun, Turkey
3Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
4Department of Pathology, Faculty of Veterinary, Sivas Cumhuriyet University, Sivas, Turkey
5Institute of Science, Ataturk University, Erzurum, Turkey
6Department of Medical Genetics, Erzincan Binali Yıldırım University, Erzincan, Turkey
7Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey
8Department of Medical Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
DOI : 10.29228/jrp.411 Products from Mentha species are widely used in medical applications, including wound healing, due to the bioactive compounds they may contain. However, data on the effect of Mentha longifolia L. on wound healing are limited. This study investigated the antimicrobial and wound healing-promoting effects of Mentha longifolia L. essential oil using in vitro methods. The chemical compositions of the essential oil were identified using gas chromatography/mass spectrometry, while the agar well diffusion and disk diffusion methods were used to determine antimicrobial activity against pathogenic strains. A scratch wound healing assay was performed following determination of the cytotoxic dose in human fibroblast cell lines. The media were used for biochemical analysis 48 h after the in vitro wound model. Immunohistochemical staining was applied to determine the contribution of the essential oil to wound healing. The essential oil exhibited varying levels of antimicrobial activity against the tested pathogens and increased cell viability. All doses in the scratch wound healing assay promoted wound closure in a shorter time than in the control group. TAC levels were higher in the treated groups than in the control group, while TOS, IL-6, and TNF-α levels were lower. Levels of expression of FGF 2, IGF, and TGF-β were higher in the treated groups than in the control group at increasing doses. The essential oil of Mentha longifolia L. exhibited antimicrobial effects and improved wound healing at doses of 5 μg/mL and 10 μg/mL. Keywords : Mentha longifolia L.; wound healing; fibroblast; essential oil; antimicrobial effect
Marmara University