¹Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, Kayışdağı, İstanbul, Türkiye
²Department of Toxicology, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar University, İstanbul, Türkiye
³Department of Toxicology, Faculty of Pharmacy, Yeditepe University, Kayışdağı, İstanbul, Türkiye
⁴Department of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary
⁵ELKH-USZ Biologically Active Natural Products Research Group, University of Szeged, Szeged, Hungary DOI : 10.29228/jrp.409 Using various chromatographic methods, seven compounds, including two secoiridoid glycosides; depressine (1) and gentiopicroside (2), one iridoid glycoside; loganic acid (3), two flavone-C-glycosides; isoorientin (4) and isovitexin (5), one xanthone-C-glycoside, mangiferin (6) as well as a nucleoside; adenosine (7) were isolated from the MeOH extract prepared from the aerial parts of Gentiana asclepiadea L. Their structures were elucidated unambiguously by spectroscopic methods such as 1D and 2D NMR as well as HRESIMS. The anti-inflammatory and analgesic activities of the isolated compounds were also evaluated in vitro. Among the tested compounds, 1, 2, and 4 showed potent anti-inflammatory activity through both nitrite and IL-6 pathways at 200 μM. Besides, compound 1 exhibited the highest decrease in PGE2 level, with a higher inhibition rate compared to positive control indomethacin. Keywords : Gentiana asclepiadea; Gentianaceae; secoiridoid; depressine; anti-inflammatory and analgesic activity