¹Pharmacy Services, Vocational School, Yuksek Ihtisas University, Ankara, Türkiye
²Department of Medical Pharmacology, Faculty of Medicine, Bilecik Seyh Edebali University, Bilecik, Türkiye DOI : 10.29228/jrp.399 In the present paper, a colorimetric method for the determination of tazobactam and piperacillin based on plasmonic nanoparticles (AuNPs) is suggested. Plasmonic nanoparticles have gained interest for application in the creation of sensitive analytical techniques because of their unique features. The localized surface plasmon resonance (LSPR) absorption band for AuNPs with a wavelength 543.5 (A543.5) nm was used for analysis. The working parameters such as the amount of AuNPs and pH were optimized to obtain the optimum experimental conditions. The calibration curve for the colorimetric method was prepared with tazobactam and piperacillin concentrations ranging from 2.25- 30.04 mg/L and0.52-10.33 mg/L, respectively. The detection limits were found to be 1.01 mg/L for tazobactam and 0.29 mg/L for piperacillin The AuNPs-based colorimetric method suggested in this work can use as an alternative analytical method for routine analysis of tazobactam and piperacillin because it is quick, simple, and affordable. Keywords : AuNP; piperacillin; spectrophotometry; tazobactam