Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Journal of Research in Pharmacy
2023 , Vol 27 , Issue 2
Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography
1Department of Pharmaceutic, School of Pharmacy, Institut Teknologi Bandung, Bandung, West Java, Indonesia2Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Central Java, Indonesia
3Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Institut Teknologi Bandung, Bandung, West Java, Indonesia DOI : 10.29228/jrp.371 Background: cinchonine nanostructured lipid carrier is a preparation with a nanoparticle lipid delivery system. Analysis of levels (efficiency of entrapment) of cinchonine lipid nanostructure is critical to ensure the accuracy of drug dosage. Objective: development and validation of an analytical method for the direct assay of nanostructured lipid carrier’s cinchonine modified by liquid-liquid extraction using high-performance liquid chromatography. Methodology: The research was started by determining the maximum wavelength and validation parameters of selectivity, linearity, sensitivity, precision (intraday and inter days), accuracy, and assay (entrapment efficiency) of nanostructured lipid carrier’s cinchonine directly with modification of liquid-liquid extraction. Results: maximum wavelength of cinchonine 289 nm; selectivity (RF 4.096 ± 0.30 min and TF 1); linearity (R2) 0.9998; sensitivity (LoD 1.2016 mg/L and LoQ 4.0054 mg/L); precision (RSD intraday and RSD inter days <2%); accuracy (recovery 99.52 – 99.87%), entrapment efficiency using direct assay of 94.85 ± 1.91% and indirect assay of 93.35 ± 0.22%. Conclusion: The analysis method for the direct assay of nanostructured lipid carrier’s cinchonine modified by the liquid-liquid extraction method using high-performance liquid chromatography is effective, efficient, and specific with high validity to determine the concentration (entrapment efficiency) of cinchonine from nanostructured lipid carrier’s cinchonine. Keywords : Cinchonine; Liquid-liquid extraction; Validation method; Nanostructured lipid carrier