Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 2
Additives screening for the formulation of solid lipid nanoparticles of alpha-mangostin
Nagendra Babu VUTTI1,SN Koteswara Rao GUDHANTI2,Rajasekhar Reddy ALAVALA3,Prasanna Kumar DESU1,Roja Rani BUDHA4,Kishore Babu GOVADA5,Durga Prasad ARJA5,Nagendra CH5
1College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh 522502, India
2Department of Pharmacy, School of Medical and Allied Sciences, Galgotias University, Greater Noida, Uttar Pradesh, 203201, India
3Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKM’s NMIMS, Vile Parle (W), Mumbai 400056, India
4Department of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati, A.P., 517502, India
5Department of Formulation R&D, Laila Nutraceuticals, Vijayawada, Andhra Pradesh 520007, India
DOI : 10.29228/jrp.361 The purpose of the study is to perform pre-formulation studies for alpha-mangostin (α-mangostin) and screening of additives (such as solid lipids, emulsifiers and cryoprotectants) and their combinations (ratio of lipids, ratio of drug: lipid, ratio of emulsifier: co-emulsifier and concentration of cryoprotectant) used in the formulation of solid lipid nanoparticles of α-mangostin. This screening is essential for the formulation of solid lipid nanoparticles (SLNP) that provide small particle size and PDI, high entrapment efficiency and zeta potential. This screening offers a rationale for selecting additives and their concentrations for formulating optimized SLNP. Preformulation studies showed melting point of 181.5°C, partition coefficient of 0.359 and drug solubility of 0.3072, 0.4576, 0.4892 and 0.5782 mg/mL in 1.2, 6.8, 7.0 and 7.5 pH buffers respectively. Ultimately, the DSC thermogram defines the sharp endotherm of α-mangostin at 195.98°C. Hot melt homogenization followed by ultrasonication technique is used to develop solid lipid nanoparticles. Process parameters such as homogenization speed (15,000 rpm) and ultra sonication (6 minutes) was optimized based on particle size and PDI. The optimized formulation of SLNP of α-mangostin contain 1:2:0.5:0.5:5 ratio of Drug: Solid lipid (0.8:1.2 ratio of Stearic acid: Precriol ATO5): Poloxamer 407: Sodium taurocholate: Mannitol. Keywords : α-mangostin; Pre-formulation studies; Solid lipid nanoparticles; Additives screening
Marmara University