¹Department of Medical Pharmacology, Faculty of Medicine, Istanbul Medipol University, Beykoz 34815 Istanbul, Türkiye
²Department of Histology and Embryology, Faculty of Medicine, Istanbul Medeniyet University, Kadıköy 34720 İstanbul, Türkiye
³Department of Pharmacology, Faculty of Pharmacy, University of Health Sciences, Üsküdar 34668 İstanbul, Türkiye
⁴Department of Pharmacognosy, Faculty of Pharmacy, Istanbul Medipol University, Beykoz 34815 Istanbul, Türkiye
⁵Department of Histology and Embryology, Faculty of Medicine, Istanbul Medipol University, Beykoz 34815 Istanbul, Türkiye
⁶Department of Medical Pharmacology, Faculty of Medicine, Istanbul Medipol University, Beykoz 34815 Istanbul, Türkiye
⁷Department of Animal Facility, Research Institute for Health Sciences and Technologies, Istanbul Medipol University, Beykoz 34815 Istanbul, Türkiye
⁸Department of Physiology, Faculty of Medicine, Alanya Alaaddin Keykubat University, Alanya 07450 Antalya, Türkiye DOI : 10.29228/jrp.349 Carduus adpressus has been used for its anti-hair loss effect in traditional folk medicine. The plant species is mainly distributed in Türkiye, Bulgaria, and the Western Caucasus. The studies on this specific plant in the genus Carduus is limited which remarks the significance of the current study. This study aims to investigate the antibacterial, antioxidant, and wound-healing properties of the methanolic extract of Carduus adpressus. Extract was obtained by maceration. The broth microdilution assay was performed on Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. DPPH and ABTS radical scavenging assays were performed to detect the antioxidant capacity. Wound-healing activity was tested using alloxan-induced diabetic BALB-c mice. 4 groups, control, vehicle, CAE and Carduus adpressus groups were treated with the relative agent for 10 days. Extract demonstrated 62.5 μg/mL MIC against S. aureus and E. faecalis, and 125 μg/mL MIC against P. aeruginosa and E. coli. ABTS assay showed higher antioxidant activity compared to the DPPH assay. Carduus adpressus group demonstrated strong regeneration, epithelisation, and angiogenesis compared to the control group on day 10. Additionally, expression of PDGF, VEGF, and collagen formation was increased in the Carduus adpressus group compared to the control group on day 10. Extract demonstrated strong antibacterial, antioxidant, and wound-healing activities which indicate that it could be a source in developing wound-healing agents. Keywords : Antibacterial; antioxidant; Carduus adpressus; extract; BALB-c mice; wound-healing