Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 1
The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS
1Institute of Science, Marmara University, 34854, Maltepe, Istanbul, Türkiye
2Department of Analytical Chemistry, Marmara University, Faculty of Pharmacy, Basıbuyuk Istanbul Türkiye
3Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Türkiye
DOI : 10.29228/jrp.313 Ellagic acid (EA) and resveratrol (RES) are phenolic compounds naturally found in fruits. However, a significant amount of RES and EA is present in berries and their effective isolation enables the production of functional foods. In this study, EA and RES found in four highbush (Vaccinium corymbosum) blueberry varieties (Bluecrop, Brigitta, Darrow and Bluejay) were analyzed simultaneously for the first time. A chromatographic method was developed for EA and RES by HPLC-DAD and LC- MS/MS. Furthermore, pressurized liquid extraction (PLE) method was performed for the extraction of EA and RES from blueberry samples. LOD (limit of detection) and LOQ (limit of quantification) were determined as 0.1898x10-6 mol.L-1 and 0.5694x10-6 mol.L-1 for RES, 0.1271x10-6 mol.L-1 and 0.3814x10-6 mol.L-1 for EA, respectively. All the results were reevaluated according to 91.85% and 84.97% recovery value, for EA and RES, respectively. The amount of EA and RES were found out in the range of 1.65 - 9.16 mg/kg and 2.95 - 9.31 mg/kg in the lyophilized blueberry varieties. Keywords : Blueberry; ellagic acid; resveratrol; LC-MS/MS; HPLC
Marmara University