Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 1
Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma
Fulchan ALI1,Sk HABIBULLAH2,Yashwant GIRI3,Amulyaratna BEHERA3,Biswaranjan MOHANTY2
1Research Scholar, Biju Patnaik University of Technology, Rourkela, Odisha, India
2Institute of Pharmacy & Technology, Salipur, Odisha, India
3School of Pharmacy and Life Sciences, Faculty of Pharmacy, Centurion University of Technology and Management, Odisha, India
DOI : 10.29228/jrp.291 The present research work was proposed to develop a pH triggered in-situ gel formulation for the ocular delivery of acetazolamide to treat glaucoma. Carbopol-934, Carbopol 940, Hydroxypropyl methylcellulose (HPMC) K4M, and Sodium Alginate were chosen for the development of in-situ gel. Formulation with Carbopol 940 and sodium alginate was optimized by assessing the gelling capacity. The prepared in-situ gel formulations were thoroughly characterized for gelling time, gelation temperature, Fourier-transform infrared (FTIR) spectroscopy studies, rheological study, sterility testing, corneal drug permeation, ocular irritation test, and accelerated stability study. Ex-vivo corneal permeation study was performed using goat cornea. The result of transcorneal permeation of acetazolamide followed the Fickian diffusion process. The optimized formulations showed satisfactory gelling time (4.17-5.17 sec) and dissolution time (120 min). FTIR study confirms the compatibility between the polymers and acetazolamide. The sterility study showed a satisfactory result. After 72 h observation in the in-vivo rabbit eye irritation study, the eyes appeared normal. There were no significant changes in pH and drug content in the accelerated stability studies of the formulations. Keywords : Acetazolamide; gelation time; carbopol-934&940; sodium alginate; in-situ gel; release; viscosity
Marmara University