¹Master Program of Tropical Medicine, Faculty of Medicine, Airlangga University, Surabaya City, 60132, Indon
²Department of Biology, Faculty of Information Technology and Science, Hindu University of Indonesia, Denpasar City, 80236, Indonesia DOI : 10.29228/jrp.271 Long-term control of Aedes aegypti larvae with temephos has led to resistance and side effects. Therefore, natural agents have been developed to eradicate the vector that causes dengue fever. Therefore, the aim of this study is to evaluate the efficacy and toxicity of Parasayu incense ash as a larvicide to eradicate Aedes aegypti mosquito larvae. In this experimental study, a completely randomized design was used and the test was conducted by exposing 25 fourth instar larvae to Parasayu incense ash for two, four, six, eight and 24 hours, with five replicates for a total sample of 875 tails in the Entomology and Parasitology Laboratory for three months. Data were analyzed using one-way ANOVA and a probit test. One test showed that different exposure times to Parasayu incense ash significantly affected the death of Aedes aegypti mosquito larvae at p = 0.000 (p< 0.01). The ash toxicity test yielded a lethal time (LT50) value of 57.4539, which is 5 h, 37 min, and 32 s in the extremely hazardous category. In addition, the LT90 value was 4.632736, which is 3 h, 21 min, and 48 s in the super toxic category. The coefficient of determination (R2) was 0.9552 (95.52%), and the correlation coefficient (r) was 0.9773 (97.73%). Therefore, to eradicate the vector causing dengue virus infection, Parasayu incense ash should be used, which has been shown to be effective, efficient, and toxic to Aedes aegypti mosquito larvae at exposure times of 2, 4, 6, 8, and 24 hours. Keywords : Aedes aegypti; Ash; Dengue virus; Infectious disease vector; Parasayu incense; Larvicides