Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 6
LC-MS/MS analysis and biological activities of endemic Achillea sieheana Stapf from Türkiye
1Department of Pharmacognosy, Faculty of Pharmacy, Marmara University, Istanbul, Türkiye
2Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Türkiye
DOI : 10.29228/jrp.270 In this study, in vitro ABTS, DPPH, 5-lipoxygenase, and α-glucosidase inhibitory activities of n-hexane (ASH), dichloromethane (ASD), ethyl acetate (ASE), and methanol (ASM) extracts from the aerial parts of endemic A. sieheana were assayed for the first time. The phytochemical content of ASE, the most active extract, has been first determined by LC-MS/MS. Also, the total amount of phenolic compounds of extracts were calculated.

ASE demonstrated significant antioxidant activity with IC50 values of 0.096 and 0.156 mg/mL for ABTS and DPPH, respectively. ASD and ASE with IC50 values of 0.045 and 0.089 mg/mL exhibited significant anti-inflammatory activity. ASE showed moderate α-glucosidase inhibitory activity with an IC50 value of 0.774 mg/mL. Also, the total phenolic content was found to be highest in the ASE. Feruloylquinic acid, luteolin, luteolin glucoside, isorhamnetin, isovitexin, methoxyflavonoid like chrysoeriol and its glycoside derivative in the ASE were detected by LC-MS/MS. The results demonstrated that ASE had significant anti-inflammatory, antioxidant and moderate α-glucosidase inhibitory potential. Keywords : A. sieheana; LC-MS/MS; antioxidant; anti-inflammatory; α-glucosidase

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