¹Institute of Animal Biology NAAS, Lviv, Ukraine DOI : 10.29228/jrp.224 Carbon tetrachloride (CCl₄) causes tissues damage by inducing a state of oxidative stress. This research was arranged to evaluate the role of L-glutamic acid (L-Glu) against oxidative stress induced by CCl₄ in brain and spleen tissues of rats. Antioxidant profiles were evaluated by estimating the activities of antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and reduced glutathione (GSH). In addition to this, the lipid peroxidation products content (TBARS and LOOH) was monitored. Rats were divided into three groups: two experimental (CCl₄, CCl4/L-Glu) and Control. The rats of CCl₄ group were treated with CCl4 only, CCl4/L-Glu group was treated with CCl₄ and L-Glu. Administration of CCl₄ reduced the antioxidant enzymes activity in the spleen and brain tissues of rats. Co-treatment of L-Glu restored the activities of antioxidant enzymes GPx (brain), GST (spleen), GR (spleen, brain) compared to control and CCl₄ experimental group of animals. Increased spleen TBARS content was observed in rats treated with CCl₄. But this index didn't change in the CCl₄/L-Glu group, which additionally received L-Glu. Results of the present study revealed that LGlu could protect the brain and spleen tissues against oxidative stress induced by CCl₄. Keywords : L-glutamic acid; carbon tetrachloride; antioxidant system; enzymes; oxidative stress