¹Department of Pharmaceutical Sciences, Faculty of Pharmacy, Zarqa University, Zarqa, Jordan
²Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman, Jordan
³Department of Clinical Pharmacy and Therapeutics, Faculty of Pharmacy, Applied Science Private University, Amman, Jordan
⁴Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, Jordan DOI : 10.29228/jrp.223 Medicinal plants are a rich source of disease-prevention medicinal chemicals, and their health advantages are continually expanding. Traditional treatments can help with a variety of disease conditions, but they need to be scientifically validated before they can be fully utilized. The current study was therefore conducted to evaluate the diuretic activity of ethanolic extract of Teucrium polium L. in Wistar rats. The ground aerial parts of T. polium were extracted with 70% ethanol in a Soxhlet apparatus. The Wistar rats were divided into four groups; one control group and three treatment groups that were given a reference diuretic medication (furosemide at 10 mg/kg), and ethanolic extracts of T. polium (200 and 400 mg/kg). 5 hours after the administration, the volume of urine and electrolytes (Na+, K+, and Cl) excretion were calculated. Also, diuretic index, diuretic, and saluretic activities as well as carbonic anhydrase inhibition were estimated. The results showed that the 200 and 400 mg/kg treatment groups had a significant increase (P < 0.05) in urine volume and mild diuretic activity of 0.78 and 0.99, respectively. Compared to the control group, T. polium (400 mg/kg) ethanolic extract showed a significant increase (P < 0.05) in the excretion of K+ and Cl-. Also, there was an increase in saluretic activity in the 400 mg/kg treatment group, as measured by the Na++K+, to be 528.0, compared to the control, which had a saluretic activity of 308.0. The study supports the use of T. polium as a diuretic in traditional folk medicine. Keywords : Teucrium polium; Diuretic activity; Urine volume; Furosemide; In vivo study