Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 4
Fullerene modified single-use electrodes as a convenient biosensor platform for electrochemical monitoring of drug- DNA interaction
1Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, 35100, Izmir Turkey
2Biomedical Device Technology Program, Vocational School of Health Services, Izmir Democracy University, Izmir 35140, Turkey
DOI : 10.29228/jrp.197 Fullerene enriched disposable electrodes were developed and used as a biosensor platform for electrochemical monitoring of drug-DNA interaction. The surface of pencil graphite electrode (PGE) was modified with fullerene in order to enhance electrode surface for efficient dsDNA immobilization. The surface of fullerene modified PGEs (f-PGEs) was characterized using scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS). The behavior of dsDNA layer onto f-PGE surface was investigated by EIS measurements in Fe(CN6)4-/3- redox system. After optimization of several experimental parameters, the suitability of the constructed electrodes as a sensor platform for monitoring of drug-DNA interaction has been examined. The reproducibility of the biosensor was also tested in the presence of dsDNA immobilization. Consequently, the developed fullerene based biosensor offers suitable platform for the electrochemical analysis of drug-DNA interaction sensitively. Keywords : Fullerene; pencil graphite electrodes; electrochemical biosensor; electrochemical impedance spectroscopy; drug-DNA interaction
Marmara University